Please use this identifier to cite or link to this item: http://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/2172
Title: Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome
Authors: Zapate Benavides, Pablo
Arellano Rodríguez, Mariela
Bollain y Goytia, Juan José
Franco Molina, Moisés Armides
Rangel Ochoa, Gloria Azucena
Avalos Díaz, Esperanza del Refugio
Herrera Esparza, Rafael
Rodríguez Padilla, Cristina
Issue Date: Feb-2017
Publisher: Hindawi
Abstract: Nephrotic syndrome (NS) is a glomerular disease that is defined by the leakage of protein into the urine and is associated with hypoalbuminemia, hyperlipidemia, and edema. Steroid-resistant NS (SRNS) patients do not respond to treatment with corticosteroids and show decreased Wilms tumor 1 (WT1) expression in podocytes. Downregulation of WT1 has been shown to be affected by certain microRNAs (miRNAs). Twenty-one patients with idiopathic NS (68.75% were SSNS and 31.25% SRNS) and 10 healthy controls were enrolled in the study. Podocyte number and WT1 location were determined by immunofluorescence, and the serum levels of miR-15a, miR-16-1, and miR-193a were quantified by RT-qPCR. Low expression and delocalization of WT1 protein from the nucleus to the cytoplasm were found in kidney biopsies of patients with SRNS and both nuclear and cytoplasmic localization were found in steroid-sensitive NS (SSNS) patients. In sera from NS patients, low expression levels of miR-15a and miR-16-1 were found compared with healthy controls, but only the miR-16-1 expression levels showed statistically significant decrease (𝑝 = 0.019). The miR-193a expression levels only slightly increased in NS patients. We concluded that low expression and delocalization from the WT1 protein in NS patients contribute to loss of podocytes while modulation from WT1 protein is not associated with the miRNAs analyzed in sera from the patients.
URI: http://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/2172
https://doi.org/10.48779/hq9z-9497
ISSN: 2314-6141
Other Identifiers: info:eu-repo/semantics/publishedVersion
Appears in Collections:*Documentos Académicos*-- UA Medicina

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