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Title: Olfactomedin‑like 2 A and B (OLFML2A and OLFML2B) expression profile in primates (human and baboon)
Authors: Pérez Ibave, Diana Cristina
González Álvarez, Rafael
Martínez Fierro, Margarita de la Luz
Ruíz Aima, Gabriel
Luna Muñoz, Maricela
Martínez de Villareal, Laura Elía
Garza Rodríguez, María de Lourdes
Reséndez Pérez, Diana
Mohamed Noriega, Jibran
Garza Guajardo, Raquel
Bautista de Lucio, Victor Manuel
Mohamed Noriega, Karim
Barbosa Quintana, Oralia
Arámburo de la Hoz, Carlos
Barrera Saldaña, Hugo Alberto
Rodríguez Sánchez, Iram Pablo
Issue Date: Nov-2016
Publisher: BioMed Central
Abstract: Background: The olfactomedin‑like domain (OLFML) is present in at least four families of proteins, including OLFML2A and OLFML2B, which are expressed in adult rat retina cells. However, no expression of their orthologous has ever been reported in human and baboon. Objective: The aim of this study was to investigate the expression of OLFML2A and OLFML2B in ocular tissues of baboons (Papio hamadryas) and humans, as a key to elucidate OLFML function in eye physiology. Methods: OLFML2A and OLFML2B cDNA detection in ocular tissues of these species was performed by RT‑PCR. The amplicons were cloned and sequenced, phylogenetically analyzed and their proteins products were confirmed by immunofluorescence assays. Results: OLFML2A and OLFML2B transcripts were found in human cornea, lens and retina and in baboon cornea, lens, iris and retina. The baboon OLFML2A and OLFML2B ORF sequences have 96% similarity with their human’s orthologous. OLFML2A and OLFML2B evolution fits the hypothesis of purifying selection. Phylogenetic analysis shows clear orthology in OLFML2A genes, while OLFML2B orthology is not clear. Conclusions: Expression of OLFML2A and OLFML2B in human and baboon ocular tissues, including their high simi‑ larity, make the baboon a powerful model to deduce the physiological and/or metabolic function of these proteins in the eye.
ISSN: 0717-6287
Other Identifiers: info:eu-repo/semantics/publishedVersion
Appears in Collections:*Documentos Académicos*-- Doc. en Ing. y Tec. Aplicada

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