Elizondo Zertuche, Mariana; M. Montoya, Alexandra; Robledo Lea, Efrén; Garza Veloz, Idalia; Sánchez Núñez, Ana L.; Ballesteros Elizondo, Raquel; M. González, Gloria
Resumen:
We identified 11 Lomentospora prolificans
isolates recovered from Mexican patients using phenotypic
and molecular characteristics. The identification
of isolates was assessed by internal transcribed
spacer (ITS rDNA) sequencing. In vitro susceptibility
to amphotericin B, fluconazole, voriconazole,
posaconazole, caspofungin, anidulafungin and micafungin
was determined according to Clinical and
Laboratory Standards Institute (CLSI) procedures.
Three isolates (07-2239, 11-2242 and 04-2673) were
used to induce systemic infection in immunocompetent
ICR mice. Survival and tissue burden studies were
used as markers of pathogenicity. All of the strains
were resistant to every antifungal tested with MIC’s for
AmB (8–[8 lg/ml), VRC (16–[16 lg/ml), PSC (16–
[16 lg/ml), FLC (64–[64 lg/ml) and echinocandins
with MICs C8 lg/ml. One hundred, ninety and sixty
percent of the infected mice with the strains 07-2239,
11-2242 and 04-2673 died during the study, respectively.
Regarding tissue burden, the highest fungal load
of the infected mice was detected in brain followed by
spleen and kidney, regardless of the strain.