Martínez Fierro, Margarita de la Luz; Garza Veloz, Idalia; Gutierrez Arteaga, Christina; Delgado Enciso, Iván; Barbosa Cisneros, Olga Yadira; Flores Morales, Virginia; Hernández Delgadillo, Gloria Patricia; Rocha Pizaña, María del Refugio; Rodríguez Sánchez, Iram Pablo; Badillo Almaráz, José Isaías; Ortíz Rodríguez, José Manuel; Castañeda Miranda, Rodrígo; Solís Sánchez, Luis Octavio; OrtÍz Castro, Yolanda
Resumen:
Purpose: To perform serum microRNA expression profiling to identify members of chromosome 19 miRNA cluster involved in preeclampsia development.
Methods: Serum chromosome 19 miRNA cluster microRNA expression profiling was evaluated at 12, 16, and 20 gestational weeks and at the time of preeclampsia diagnosis, in women who developed preeclampsia (WWD-PE; n = 16) and controls (n = 18) using TaqMan low density array plates.
Results: A total of 51 chromosome 19 microRNA cluster members were evaluated. The circulating hsa-miRs 512-3p, 518f3p, 520c-3p, and 520d-3p, were differentially expressed between groups (P < 0.05). Compared with controls, serum levels of hsa-miR-518f-3p at 20 GW were useful for identifying WWD-Mild-PE (P = 0.035) and WWD-Severe-PE(P = 0.007).
Conclusions: Serum hsa-miRs 512-3p, 518f-3p, 520c-3p, and 520d-3p, are differentially expressed between WWD-PE and controls and their role in the development of preeclampsia should be investigated further.