Luna Aguirre, Claudia Maribel; Martínez Fierro, Margarita de la Luz; Mar Aguilar, Fermín; Garza Veloz, Idalia; Treviño Alvarado, Víctor; Rojas Martínez, Augusto; Jaime Pérez, José Carlos; Malagon Santiago, Guadalupe Ismael; Gutiérrez Aguirre, César Homero; González Llano, Oscar; Salazar Riojas, Rosario; Hidalgo Miranda, Alfredo; Martínez Rodríguez, Herminia Guadalupe; Gómez Almaguer, David; Ortíz López, Rocío
Resumen:
BACKGROUND: Acute lymphoblastic leukemia (ALL) is a highly diverse disease characterized by cytogenetic and molecularabnormalities, including altered microRNA (miRNA) expression signatures.
AIM: We perform and validate a plasma miRNA expression profiling to identify potential miRNA involved in leukemogenesis
METHODS: MiRNA expression profiling assay was realized in 39 B-ALL and 7 normal control plasma samples using TaqMan Low Density Array (TLDA) plates on Applied Biosystems 7900 HT Fast Real-Time PCR System. MiRNA validation was done for six miRNA differentially expressed by quantitative real-time PCR.
RESULTS: Seventy-seven circulating miRNA differentially expressed: hsa-miR-511, -222, and -34a were overexpressed, whereas hsa-miR-199a-3p, -223, -221, and -26a were underexpressed (p values < 0.005 for both sets). According to operating characteristic curve analysis, hsa-miR-511 was the most valuable biomarker for distinguishing B-ALL from normal controls,with an area under curve value of 1 and 100% for sensitivity, and specificity respectively.
CONCLUSIONS: Measuring circulating levels of specific miRNA implicated in regulation of cell differentiation and/or cell proliferation such as hsa-miRNA-511, offers high sensitivity and specificity in B-ALL detection and may be potentially useful for detection of disease progression, as indicator of therapeutic response, and in the assessment of biological and/or therapeutic targets for patients with B-ALL.