Please use this identifier to cite or link to this item: http://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/2177
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dc.contributor4495es_ES
dc.contributor84378es_ES
dc.contributor242078es_ES
dc.contributor243523es_ES
dc.contributor4494es_ES
dc.contributor.otherhttps://orcid.org/0000-0002-3403-9849-
dc.coverage.spatialGlobales_ES
dc.creatorRodríguez Rodríguez, Mayra-
dc.creatorHerrera Esparza, Rafael-
dc.creatorBollain y Goytia, Juan José-
dc.creatorPérez Pérez, María Elena-
dc.creatorPacheco Tovar, Deyanira-
dc.creatorMurillo Vázquez, Jessica-
dc.creatorPacheco Tovar, Guadalupe-
dc.creatorAvalos Díaz, Esperanza del Refugio-
dc.date.accessioned2020-12-05T05:04:49Z-
dc.date.available2020-12-05T05:04:49Z-
dc.date.issued2017-11-26-
dc.identifierinfo:eu-repo/semantics/publishedVersiones_ES
dc.identifier.issn23147156es_ES
dc.identifier.issn23148861es_ES
dc.identifier.urihttp://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/2177-
dc.identifier.urihttps://doi.org/10.48779/cj7v-8805-
dc.description.abstractThe goal of the present study was to determine whether peptidylarginine deiminase PAD2 and PAD4 enzymes are present in Balb/c mouse salivary glands and whether they are able to citrullinate Ro and La ribonucleoproteins. Salivary glands from Balb/c mice were cultured in DMEM and supplemented with one of the following stimulants: ATP, LPS, TNF, IFNγ, or IL-6. A control group without stimulant was also evaluated. PAD2, PAD4, citrullinated peptides, Ro60, and La were detected by immunohistochemistry and double immunofluorescence. PAD2 and PAD4 mRNAs and protein expression were detected by qPCR and Western blot analysis. PAD activity was assessed using an antigen capture enzyme-linked immunosorbent assay. LPS, ATP, and TNF triggered PAD2 and PAD4 expression; in contrast, no expression was detected in the control group (p < 0 001). PAD transcription slightly increased in response to stimulation. Additionally, PAD2/4 activity modified the arginine residues of a reporter protein (fibrinogen) in vitro. PADs citrullinated Ro60 and La ribonucleoproteins in vivo. Molecular stimulants induced apoptosis in ductal cells and the externalization of Ro60 and La ribonucleoproteins onto apoptotic membranes. PAD enzymes citrullinate Ro and La ribonucleoproteins, and this experimental approach may facilitate our understanding of the role of posttranslational modifications in the pathophysiology of Sjögren’s syndrome.es_ES
dc.language.isoenges_ES
dc.publisherHindawies_ES
dc.relationhttps://www.hindawi.com/journals/jir/2017/8959687/es_ES
dc.relation.urigeneralPublices_ES
dc.rightsAtribución-NoComercial-CompartirIgual 3.0 Estados Unidos de América*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.sourceJournal of Immunology Research Vol. 2017, pp. 1-15es_ES
dc.subject.classificationMEDICINA Y CIENCIAS DE LA SALUD [3]es_ES
dc.subject.otherPAD2es_ES
dc.subject.otherPAD4es_ES
dc.subject.otherenzymeses_ES
dc.titleActivation of Peptidylarginine Deiminase in the Salivary Glands of Balb/c Mice Drives the Citrullination of Ro and La Ribonucleoproteinses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
Appears in Collections:*Documentos Académicos*-- UA Medicina

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