Evaluación por medio de intradermorreacción de la aplicación del inmunógeno de 45kDa de Trichinella spiralis para el diagnóstico de Trichinellosis

Abstract

Diagnostic methods for different zoonotic diseases in the food industry have helped to control, treat and eradicate several food-transmitted diseases such as Trichinellosis, a parasitic disease mainly transmitted through consumption of poorly cooked meat, that is not commonly detected in any type of meat processing facility. The main objective of this work is to evaluate the delayed hypersensitivity immunological mechanism by administration of the 45 kDa Trichinella spiralis immunogen in order to develop an easy access and costeffective diagnostic method for the detection of the disease both in humans and animals. In this study, Long Evans murines were distributed in six different groups: healthy and infected control groups, murinestreated sublingually and conventionally with the 45 kDa immunogene after infection with T. spiralis, and murines challenged with 500 infective larvae after sublingual and conventional immunization. The intradermal reaction was performed using the 45 kDa immunogen in order to evaluate the delayed hypersensitivity by the triple response of Lewis after 2, 6, 24 and 48 h. The statistical analysis performed, consisting in a multifactorial analysis of variance, allowed us to detect significant differences between the groups considered (p < 0.05, CI: 95%), which led us to conclude that the 45 kDa antigen is a protein potentially usable for the diagnosis of Trichinellosis.