http://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/1979 2026-10-15T19:51:07Z http://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/4455 Robust Comparison of Raman Spectra Under Various Perturbations: Benchmarking Common Similarity Metrics and a New Peak-Oriented Index Reyes-Reyes, Adan; Reyes-Reyes, Juan Andres; Martínez-Ruíz, Erick Osvaldo; Ortiz-Dosal, Alejandra; Rodríguez-Aranda, Ma. del Carmen; Reyes-Reyes, Daniel Carlos; Villada-Gil, Stiven; Ortiz-Dosal, Luis Carlos; Kolosovas-Machuca, Eleazar Samuel Matching Raman spectra is complicated by shifts in band position, changes in intensity and linewidth, the appearance or loss of bands, and noise because different similarity measures preserve different forms of spectral evidence. We introduce 𝑆𝐽, a peak-oriented index based on one-to-one band assignment and explicit penalties for unmatched peaks, and evaluate it together with pointwise, rigid-alignment, elastic-alignment, and derivative-based measures. The benchmark comprises 500 baseline-corrected reference spectra and 50,000 controlled perturbed queries. Each noisy query was independently converted to a peak table before 𝑆𝐽 evaluation. Parameter sensitivity was assessed on 350 calibration references, after which the selected 𝑆𝐽 configuration and a single hybrid-ranking weight were fixed for 150 held-out references. The moderate association between 𝑆𝐽 and the waveform metrics (𝜌𝑠=0.28–0.48) indicates that band correspondence retains information not captured by waveform agreement alone. In full-library retrieval, standalone 𝑆𝐽 attained a held-out Top-1 recovery of 0.9603. Combining 𝑆𝐽 with cosine similarity, Pearson correlation, cross-correlation, or cross-cosine similarity using the same calibration-selected weight (𝑤=0.50) increased their respective Top-1 recovery rates from 0.4166 to 0.8828, 0.3960 to 0.9094, 0.9449 to 0.9930, and 0.9523 to 0.9933; reference-level bootstrap intervals for all four changes excluded zero. These results establish 𝑆𝐽 as a complementary structural layer that can strengthen both shift-sensitive and shift-tolerant library rankings, rather than as a universal replacement for waveform metrics. 2026-10-09T00:00:00Z http://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/4294 Raman and SERS Spectra of Human Myelin Basic Protein in Cerebrospinal Fluid Bravo-Oro, Antonio; Ugarte-Anchondo, Sergio; Martínez-Ruiz, Erick Osvaldo; Rodríguez-Aranda, Ma. del Carmen; Reyes-Reyes, Adán; García-Mendoza, Cristian Israel; Ortiz-Dosal, Luis Carlos; Rivera-Pérez, Emmanuel; Reyes-Reyes, Juan Andrés; Kolosovas-Machuca, Eleazar Samuel; Ortiz-Dosal, Alejandra Raman spectroscopy (RS) provides detailed information on molecular structure but remains challenging for low-scattering proteins in complex media. Myelin basic protein (MBP) is a key structural component of central nervous system myelin and a clinically relevant molecule in demyelinating disorders; however, to the best of our knowledge, its Raman signature in solution has not been reported. In this work, Raman and surface-enhanced Raman spectroscopy (SERS) were employed to characterize purified human myelin basic protein (MBP) in aqueous solution and cerebrospinal fluid (CSF). Quasi-spherical silver nanoparticles were used as SERS elements, yielding enhancement factors of 105 and increasing sensitivity to MBP-associated spectral changes at low concentrations. The MBP spectrum exhibited vibrational modes primarily associated with amide II and amide III bands, as well as aromatic side-chain contributions. Comparative analysis of MBP, CSF, and MBP-spiked CSF samples revealed significant spectral overlap, limiting discrimination based solely on peak positions. To overcome this limitation, spectral correlation and band-intensity-ratio analyses were applied, revealing reproducible trends associated with increasing MBP content. While individual MBP bands are not exclusive, the observed spectral patterns demonstrate the sensitivity of RS and SERS to MBP-induced spectral changes in CSF. These findings should be interpreted as a proof-of-concept in a single-donor CSF matrix. Raman spectroscopy (RS) provides detailed information on molecular structure but remains challenging for low-scattering proteins in complex media. Myelin basic protein (MBP) is a key structural component of central nervous system myelin and a clinically relevant molecule in demyelinating disorders; however, to the best of our knowledge, its Raman signature in solution has not been reported. In this work, Raman and surface-enhanced Raman spectroscopy (SERS) were employed to characterize purified human myelin basic protein (MBP) in aqueous solution and cerebrospinal fluid (CSF). Quasi-spherical silver nanoparticles were used as SERS elements, yielding enhancement factors of 105 and increasing sensitivity to MBP-associated spectral changes at low concentrations. The MBP spectrum exhibited vibrational modes primarily associated with amide II and amide III bands, as well as aromatic side-chain contributions. Comparative analysis of MBP, CSF, and MBP-spiked CSF samples revealed significant spectral overlap, limiting discrimination based solely on peak positions. To overcome this limitation, spectral correlation and band-intensity-ratio analyses were applied, revealing reproducible trends associated with increasing MBP content. While individual MBP bands are not exclusive, the observed spectral patterns demonstrate the sensitivity of RS and SERS to MBP-induced spectral changes in CSF. These findings should be interpreted as a proof-of-concept in a single-donor CSF matrix. 2026-05-12T00:00:00Z http://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/4230 Dependence of ATP content in the formation of protrusions in DMPC GUVs under an AC electric field Ángeles Robles, Gabriela; Ruiz-García, Jaime; Méndez, J. Alfredo; Ortiz-Dosal, Luis Carlos The cytoskeleton is an essential cell component. Many cellular processes that require changes in the cell structure depend on it. One of these processes is cell migration, which occurs through the formation of structures called protrusions that interact with other cytoskeletal components, enabling the cell to move slowly. These structures are formed by the polymerization of actin monomers, a process that requires the presence of ATP, as well as the exchange of divalent cations. In this work, we present a study on the formation of actin protrusions within DMPC giant unilamellar vesicles by varying the concentration of ATP, both in the absence and presence of MgCl2. It was found that when the concentration of ATP in the overall protein buffer increases, these structures form and extend inside the vesicle without breaking it, even in the absence of MgCl2. These protrusions are randomly oriented; however, when an alternating current electric field is applied, the protrusions align in the direction of the field in response to the polar nature of both lipids and actin filaments. The cytoskeleton is an essential cell component. Many cellular processes that require changes in the cell structure depend on it. One of these processes is cell migration, which occurs through the formation of structures called protrusions that interact with other cytoskeletal components, enabling the cell to move slowly. These structures are formed by the polymerization of actin monomers, a process that requires the presence of ATP, as well as the exchange of divalent cations. In this work, we present a study on the formation of actin protrusions within DMPC giant unilamellar vesicles by varying the concentration of ATP, both in the absence and presence of MgCl2. It was found that when the concentration of ATP in the overall protein buffer increases, these structures form and extend inside the vesicle without breaking it, even in the absence of MgCl2. These protrusions are randomly oriented; however, when an alternating current electric field is applied, the protrusions align in the direction of the field in response to the polar nature of both lipids and actin filaments. 2026-04-13T00:00:00Z http://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/4213 Calculando la gloria: la física detrás de la toma de Zacatecas Ángeles Robles, Gabriela; Ortiz Dosal, Luis Carlos La Revolución Mexicana, un conflicto armado que tuvo lugar entre 1910 y 1920, producto de la desigualdad social, el despojo de la tierra a los campesinos, la creación de latifundios y el ejercicio del poder dictatorial del General Porfirio Díaz, entre otras causas, fue un evento trascendental en la historia de México. Durante este período tumultuoso, diferentes facciones y líderes políticos lucharon por el poder y la justicia social en el país. Una de las figuras clave en este conflicto fue la División del Norte, comandada por el revolucionario Francisco Villa, quien se destacó por su habilidad táctica y el uso estratégico de la artillería por parte del General Felipe Ángeles 2025-11-17T00:00:00Z