http://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/1979 2026-04-23T12:12:56Z 2026-04-23T12:12:56Z http://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/4230 2026-04-16T19:48:40Z 2026-04-13T00:00:00Z

Título : Dependence of ATP content in the formation of protrusions in DMPC GUVs under an AC electric field Authors: Ángeles Robles, Gabriela; Ruiz-García, Jaime; Méndez, J. Alfredo; Ortiz-Dosal, Luis Carlos Resumen : The cytoskeleton is an essential cell component. Many cellular processes that require changes in the cell structure depend on it. One of these processes is cell migration, which occurs through the formation of structures called protrusions that interact with other cytoskeletal components, enabling the cell to move slowly. These structures are formed by the polymerization of actin monomers, a process that requires the presence of ATP, as well as the exchange of divalent cations. In this work, we present a study on the formation of actin protrusions within DMPC giant unilamellar vesicles by varying the concentration of ATP, both in the absence and presence of MgCl2. It was found that when the concentration of ATP in the overall protein buffer increases, these structures form and extend inside the vesicle without breaking it, even in the absence of MgCl2. These protrusions are randomly oriented; however, when an alternating current electric field is applied, the protrusions align in the direction of the field in response to the polar nature of both lipids and actin filaments. Descripción : The cytoskeleton is an essential cell component. Many cellular processes that require changes in the cell structure depend on it. One of these processes is cell migration, which occurs through the formation of structures called protrusions that interact with other cytoskeletal components, enabling the cell to move slowly. These structures are formed by the polymerization of actin monomers, a process that requires the presence of ATP, as well as the exchange of divalent cations. In this work, we present a study on the formation of actin protrusions within DMPC giant unilamellar vesicles by varying the concentration of ATP, both in the absence and presence of MgCl2. It was found that when the concentration of ATP in the overall protein buffer increases, these structures form and extend inside the vesicle without breaking it, even in the absence of MgCl2. These protrusions are randomly oriented; however, when an alternating current electric field is applied, the protrusions align in the direction of the field in response to the polar nature of both lipids and actin filaments.

2026-04-13T00:00:00Z http://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/4213 2026-03-18T17:15:44Z 2025-11-17T00:00:00Z

Título : Calculando la gloria: la física detrás de la toma de Zacatecas Authors: Ángeles Robles, Gabriela; Ortiz Dosal, Luis Carlos Resumen : La Revolución Mexicana, un conflicto armado que tuvo lugar entre 1910 y 1920, producto de la desigualdad social, el despojo de la tierra a los campesinos, la creación de latifundios y el ejercicio del poder dictatorial del General Porfirio Díaz, entre otras causas, fue un evento trascendental en la historia de México. Durante este período tumultuoso, diferentes facciones y líderes políticos lucharon por el poder y la justicia social en el país. Una de las figuras clave en este conflicto fue la División del Norte, comandada por el revolucionario Francisco Villa, quien se destacó por su habilidad táctica y el uso estratégico de la artillería por parte del General Felipe Ángeles

2025-11-17T00:00:00Z http://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/4096 2026-01-21T16:46:28Z 2026-01-07T00:00:00Z

Título : Design of Functionalized Photon Sieves for the Detection of Biomarkers in Running Fluids Authors: Pastor-Villarrubia, Verónica; Canul Solis, Luis Pablo; Ortiz Dosal, Luis Carlos; Hernández-Arteaga, José Gabriel Roberto; Kolosovas-Machuca, Eleazar Samuel; Sanchez-Brea, Luis Miguel; Alda, Javier Resumen : In this work, we present the design of a prototype fluid analyzer based on photon sieves, permeable diffractive optical elements capable of focusing light through diffraction. The photon sieve comprises a spatial distribution of circular apertures patterned onto an aluminum substrate, which provides intrinsic fluid permeability and functions as either a lens or a mirror. In our approach, the aluminum surface is chemically functionalized to detect a specific biomolecular marker—human serum albumin—whose interaction with the surface induces measurable changes in the spectral reflectance. The operating wavelength is selected to maximize the reflectance contrast produced by the presence of the biomarker. The optical set-up is configured such that the light source and detector lie in the same plane when the photon sieve operates in reflection. A combined geometrical and diffractive analysis is conducted to optimize their positions. Upon detection of the biomarker, the measured signal decreases to 0.43 of its initial value prior to biomarker binding. These results highlight photon sieves as a promising platform for the development of compact, lightweight, and low-cost optical chemical sensors for running fluids.

2026-01-07T00:00:00Z http://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/4018 2025-11-26T16:49:23Z 2025-11-07T00:00:00Z

Título : Asymmetric Dimethylarginine Vibrational Spectroscopy Spectra and Density Functional Theory Model Authors: Ortiz-Dosal, Luis Carlos; Canul-Solis, Luis Pablo; Rodríguez-Aranda, Ma. del Carmen; Rivera-Pérez, Emmanuel; Ortiz-Dosal, Alejandra; Guevara, Edgar; Martínez-Ruiz, Erick Osvaldo; Spectroscopy, Raman; Ortiz-Dosal, Luis Carlos; Reyes-Reyes, Adán Resumen : NG, NG-dimethylarginine (ADMA) is an endogenous compound that acts as a competitive inhibitor of nitric oxide synthase (NOS), thereby reducing nitric oxide (NO) production and contributing to endothelial dysfunction. This dysfunction plays a pivotal role in the development of various pathological conditions, including cardiovascular disease, chronic renal failure, and diabetes. The diminished bioavailability of NO is a critical factor in the progression of these disorders, and alterations in ADMA levels have emerged as significant predictors of cardiovascular events and mortality. In this study, we investigated the molecular characteristics of ADMA using a combined approach of Raman and Fourier Transform Infrared (FT-IR) spectroscopy, complemented by computational simulations with the GaussView 5.0.8 and Gaussian 09 software suite. Experimental Raman and FT-IR spectra were acquired and compared with simulated spectra generated through Density Functional Theory (DFT) calculations. This comparative analysis enabled precise vibrational band assignments and the identification of key molecular vibrational modes, providing valuable insights into ADMA’s structural and vibrational properties. These findings establish a comprehensive spectroscopic reference for ADMA, supporting its potential application as a biomarker in clinical diagnostics.

2025-11-07T00:00:00Z